Variation ID | Product Code | Pack Size | List Price | Qty | |
---|---|---|---|---|---|
KM0050-500G | 500g | £87.30 |
This is a selective medium for the isolation of Salmonella spp. and Shigella spp. from clinical specimens and environmental samples.
This formulation was developed by Hynes through a modification of Leifsons DCA medium.
The peptone provides the required carbon, nitrogen and vitamins. Lactose is a fermentable carbohydrate and neutral red is a pH indicator. Sodium thiosulphate and ferric citrate are used to detect hydrogen sulphide production indicated by the black centred colonies of hydrogen sulphite positive organisms.
Sodium desoxycholate and sodium citrate inhibit most Gram-positive organisms.
Used for the isolation of Salmonella spp. and Shigella spp. from clinical specimens and environmental samples.
Additional Information
Shelf Life (days): 1095 days
Storage Temp (C): 10-30C
Dehydrated Medium Appearance: Fine Powder
Prepared Medium Appearance: Firm Gel
Dehydrated Medium Colour: Straw
Prepared Medium Colour: Pale orange/pink
Dehydrated PH: 7.4±0.2
Prepared PH: 7.4±0.2
Formula*g/L
Peptone: 10.0
Lactose: 10.0
Sodium desoxycholate: 5.0
Sodium citrate: 8.5
Sodium thiosulphate: 5.4
Ferric citrate: 1.0
Neutral red: 0.02
Agar: 12.0
Total: 52.0
*Adjusted/supplemented as required to meet performance requirements